Study on Effect of Vitexicarpin on the Biological Behavior of Colorectal Cancer Cells by Circ_0000419/MicroRNA-224

To investigate the effect and molecular mechanism of vitexicarpin on biological behavior colorectal cancer cells is objective study. The human epithelial cell line Caco-2 were divided into control group, low, middle high dose groups vitexicarpin, plasmid cloning DNA-circ_0000419 DNA anti-microRNA-224 anti-microRNA-224-negative vitexicarpin+small interfering-circ_0000419 group vitexicarpin+microRNA-224 group. Cell activity was detected by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay. Colony formation number plate clone experiment. Apoptosis flow cytometry. Scratch test used to detect migration distance cells. Transwell method invasive cells; protein expression western blot; levels circ_0000419 microRNA-224 real-time fluorescence quantitative polymerase chain reaction; dual-luciferase reporter assay targeting relationship between microRNA-224. After treatment with doses viability Caco- 2 decreased, colony apoptosis rate increased, level E-cadherin N-cadherin increased decreased (p<0.05). Overexpression or inhibition miR-224 viability, formation, rate, distance, number, Interference overexpression reverses effects proliferation, apoptosis, invasion targets regulation expression. Vitexicarpin can inhibit malignant circ_0000419/microRNA-224.